rat brain type i astrocyte ctx tna2 Search Results


rat cortical astrocyte cultures rat cortical astrocytes  (ATCC)
94
ATCC rat cortical astrocyte cultures rat cortical astrocytes
A. Micrograph of GFAP and BrdU stained <t>astrocytes.</t> Cells were starved for 28 h, treated for the last 24 h with 1 μM U69,593 or 1 μM MOM-Sal-B and BrdU was added for the last 4 h of treatment. Primary Abs: mouse anti-BrdU monoclonal Ab (1:10 dilution) and rabbit anti-GFAP polyclonal Ab (1:1 dilution). Secondary Ab: Green fluorescence emitting fluorescein-conjugated anti-mouse IgG Ab (1:10 dilution) and Alexa Fluor 594 goat anti-rabbit IgG (1:700 dilution). Cells were designated type-1 or type-2 based on their morphology. Arrowheads show type-1 and arrows show type-2 astrocytes in the far right panel. B. Effect of U0126 on U69,593 signaling. Cells were starved 28 h, treated for the last 24 h with 1 μM U69,593 ± 1 μM U0126 and BrdU was added for the last 4 h of treatment. In these studies ≥1100 cells were counted for each determination. N=5. **p<0.01, ***p<0.001 vs. control. †††p<0.001 vs. U69,593 without inhibitor. C. Effect of CD8-βARK-C on U69,593 and MOM-Sal-B signaling. Cells were transfected with CD8 or CD8-βARK-C cDNA, starved 28 h and treated for the last 24 h with 1 μM U69,593 or 1 μM MOM-Sal-B. BrdU was added for the last 4 h of treatment. In these studies ≥650 cells were counted for each determination. N=3. *p<0.05, **p<0.01 vs. control. †p<0.05, ††p<0.01 vs. their CD8 control in the presence of U69,593. D. Effect of β-arrestin 2 siRNA on U69,593 and MOM-Sal-B signaling. Cells were transfected with KOR cDNA +/- non-targeting or β-arrestin 2 siRNA). After 24 h on growth medium, they were starved for 28 h and treated for the last 28 h with 1 μM U69,593 or 1 μM MOM-Sal-B. In these studies ≥390cells were counted for each determination. N=3. *p<0.05, **p<0.01, ***p<0.001 vs. control. †p≤0.05, †††p<0.001 vs. non-targeting siRNA control in the presence of U69,593.
Rat Cortical Astrocyte Cultures Rat Cortical Astrocytes, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+brain+type+i+astrocyte+ctx+tna2/CTX+TNA2%3B+Astrocyte%3B+Rat/pmc02606093-63-2-11
Average 94 stars, based on 1 article reviews
rat cortical astrocyte cultures rat cortical astrocytes - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier
rat cortical astrocytes  (ATCC)
96
ATCC rat cortical astrocytes
A. Micrograph of GFAP and BrdU stained <t>astrocytes.</t> Cells were starved for 28 h, treated for the last 24 h with 1 μM U69,593 or 1 μM MOM-Sal-B and BrdU was added for the last 4 h of treatment. Primary Abs: mouse anti-BrdU monoclonal Ab (1:10 dilution) and rabbit anti-GFAP polyclonal Ab (1:1 dilution). Secondary Ab: Green fluorescence emitting fluorescein-conjugated anti-mouse IgG Ab (1:10 dilution) and Alexa Fluor 594 goat anti-rabbit IgG (1:700 dilution). Cells were designated type-1 or type-2 based on their morphology. Arrowheads show type-1 and arrows show type-2 astrocytes in the far right panel. B. Effect of U0126 on U69,593 signaling. Cells were starved 28 h, treated for the last 24 h with 1 μM U69,593 ± 1 μM U0126 and BrdU was added for the last 4 h of treatment. In these studies ≥1100 cells were counted for each determination. N=5. **p<0.01, ***p<0.001 vs. control. †††p<0.001 vs. U69,593 without inhibitor. C. Effect of CD8-βARK-C on U69,593 and MOM-Sal-B signaling. Cells were transfected with CD8 or CD8-βARK-C cDNA, starved 28 h and treated for the last 24 h with 1 μM U69,593 or 1 μM MOM-Sal-B. BrdU was added for the last 4 h of treatment. In these studies ≥650 cells were counted for each determination. N=3. *p<0.05, **p<0.01 vs. control. †p<0.05, ††p<0.01 vs. their CD8 control in the presence of U69,593. D. Effect of β-arrestin 2 siRNA on U69,593 and MOM-Sal-B signaling. Cells were transfected with KOR cDNA +/- non-targeting or β-arrestin 2 siRNA). After 24 h on growth medium, they were starved for 28 h and treated for the last 28 h with 1 μM U69,593 or 1 μM MOM-Sal-B. In these studies ≥390cells were counted for each determination. N=3. *p<0.05, **p<0.01, ***p<0.001 vs. control. †p≤0.05, †††p<0.001 vs. non-targeting siRNA control in the presence of U69,593.
Rat Cortical Astrocytes, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+brain+type+i+astrocyte+ctx+tna2/CTX+TNA2/pmc03495063-109-20-26
Average 96 stars, based on 1 article reviews
rat cortical astrocytes - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier

Image Search Results


A. Micrograph of GFAP and BrdU stained astrocytes. Cells were starved for 28 h, treated for the last 24 h with 1 μM U69,593 or 1 μM MOM-Sal-B and BrdU was added for the last 4 h of treatment. Primary Abs: mouse anti-BrdU monoclonal Ab (1:10 dilution) and rabbit anti-GFAP polyclonal Ab (1:1 dilution). Secondary Ab: Green fluorescence emitting fluorescein-conjugated anti-mouse IgG Ab (1:10 dilution) and Alexa Fluor 594 goat anti-rabbit IgG (1:700 dilution). Cells were designated type-1 or type-2 based on their morphology. Arrowheads show type-1 and arrows show type-2 astrocytes in the far right panel. B. Effect of U0126 on U69,593 signaling. Cells were starved 28 h, treated for the last 24 h with 1 μM U69,593 ± 1 μM U0126 and BrdU was added for the last 4 h of treatment. In these studies ≥1100 cells were counted for each determination. N=5. **p<0.01, ***p<0.001 vs. control. †††p<0.001 vs. U69,593 without inhibitor. C. Effect of CD8-βARK-C on U69,593 and MOM-Sal-B signaling. Cells were transfected with CD8 or CD8-βARK-C cDNA, starved 28 h and treated for the last 24 h with 1 μM U69,593 or 1 μM MOM-Sal-B. BrdU was added for the last 4 h of treatment. In these studies ≥650 cells were counted for each determination. N=3. *p<0.05, **p<0.01 vs. control. †p<0.05, ††p<0.01 vs. their CD8 control in the presence of U69,593. D. Effect of β-arrestin 2 siRNA on U69,593 and MOM-Sal-B signaling. Cells were transfected with KOR cDNA +/- non-targeting or β-arrestin 2 siRNA). After 24 h on growth medium, they were starved for 28 h and treated for the last 28 h with 1 μM U69,593 or 1 μM MOM-Sal-B. In these studies ≥390cells were counted for each determination. N=3. *p<0.05, **p<0.01, ***p<0.001 vs. control. †p≤0.05, †††p<0.001 vs. non-targeting siRNA control in the presence of U69,593.

Journal:

Article Title: KAPPA OPIOIDS PROMOTE THE PROLIFERATION OF ASTROCYTES VIA G?? AND ?-ARRESTIN 2 DEPENDENT MAPK-MEDIATED PATHWAYS

doi: 10.1111/j.1471-4159.2008.05745.x

Figure Lengend Snippet: A. Micrograph of GFAP and BrdU stained astrocytes. Cells were starved for 28 h, treated for the last 24 h with 1 μM U69,593 or 1 μM MOM-Sal-B and BrdU was added for the last 4 h of treatment. Primary Abs: mouse anti-BrdU monoclonal Ab (1:10 dilution) and rabbit anti-GFAP polyclonal Ab (1:1 dilution). Secondary Ab: Green fluorescence emitting fluorescein-conjugated anti-mouse IgG Ab (1:10 dilution) and Alexa Fluor 594 goat anti-rabbit IgG (1:700 dilution). Cells were designated type-1 or type-2 based on their morphology. Arrowheads show type-1 and arrows show type-2 astrocytes in the far right panel. B. Effect of U0126 on U69,593 signaling. Cells were starved 28 h, treated for the last 24 h with 1 μM U69,593 ± 1 μM U0126 and BrdU was added for the last 4 h of treatment. In these studies ≥1100 cells were counted for each determination. N=5. **p<0.01, ***p<0.001 vs. control. †††p<0.001 vs. U69,593 without inhibitor. C. Effect of CD8-βARK-C on U69,593 and MOM-Sal-B signaling. Cells were transfected with CD8 or CD8-βARK-C cDNA, starved 28 h and treated for the last 24 h with 1 μM U69,593 or 1 μM MOM-Sal-B. BrdU was added for the last 4 h of treatment. In these studies ≥650 cells were counted for each determination. N=3. *p<0.05, **p<0.01 vs. control. †p<0.05, ††p<0.01 vs. their CD8 control in the presence of U69,593. D. Effect of β-arrestin 2 siRNA on U69,593 and MOM-Sal-B signaling. Cells were transfected with KOR cDNA +/- non-targeting or β-arrestin 2 siRNA). After 24 h on growth medium, they were starved for 28 h and treated for the last 28 h with 1 μM U69,593 or 1 μM MOM-Sal-B. In these studies ≥390cells were counted for each determination. N=3. *p<0.05, **p<0.01, ***p<0.001 vs. control. †p≤0.05, †††p<0.001 vs. non-targeting siRNA control in the presence of U69,593.

Article Snippet: Type-1 immortalized rat cortical astrocyte cultures Rat cortical astrocytes (CTX TNA2, ATCC), were established from cultures of primary type-1 astrocytes from 1-day-old rat brain frontal cortex and grown as described ( Radany et al. 1992 ; Belcheva et al. 2003 ; Belcheva et al. 2005 ).

Techniques: Staining, Fluorescence, Control, Transfection